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. 2021 Jul 13;12:390. doi: 10.1186/s13287-021-02438-y

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 7 — MiR-100-5p inhibits osteogenesis of hBMSCs and angiogenesis of HUVECs by targeting BMPR2 and inhibiting the BMPR2/smad1/5/9 pathway. A The target gene of miR-100-5p was predicted using Targetscan 7.2. B The targeting relationship between miR-100-5p and BMPR2 was verified by a luciferase assay. C Western blotting was used to measure the expression of collagen type 1, BMPR2, SMAD1/5/9, p-SMAD1/5/9, OCN, RUNX2, ALP, OPN, and PPARγ. D Western blotting was used to measure the expression of BMPR2, SMAD1/5/9, p-SMAD1/5/9, FGF2, and VEGFA in HUVECs. E ALP staining of hBMSCs (scale bar = 100 μm). F Alizarin Red S staining of hBMSCs. G Oil Red O staining of hBMSCs (scale bar = 50 μm). H Tube formation assay of HUVECs (scale bar = 200 μm). NC, transfection with NC; siBMPR2, transfection with small interfering RNA of BMPR2; siBMPR2 + antagomiR-100-5p, transfection with small interfering RNA of BMPR2 and antagonist of miR-100-5p; NM-CTL, controls for differentiation. *P < 0.05, versus NC group; #P < 0.05, versus agomiR-100-5p group. NS P>0.05, versus NC group. All data were expressed as mean ± SEM