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. 2021 Jul 13;12:399. doi: 10.1186/s13287-021-02474-8

Fig. 1.

Fig. 1

Schematic representation of the experimental design. Umbilical cord blood CD34+ cells were expanded in a co-culture system using a mesenchymal stromal cell (MSC) feeder layer (FL) from different sources for 7 days. MSC were previously expanded using medium with either FBS or HPL. UCB cells were characterized by immunophenotyping and in vitro clonogenic assays. AT, adipose tissue; BM, bone marrow; FBS, fetal bovine serum; FL, feeder layer; HPL, human platelet lysate; TNC, total nucleated cells; UCM, umbilical cord matrix