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. 2021 Jun 30;11:714756. doi: 10.3389/fonc.2021.714756

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Copyright © 2021 Wang, Liu, Li, Li, Li and Zhu

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Hepcidin antagonist pretreatment reduces Sorafenib-induced cell death in HCC cells. HepG2 and Huh7 cells were pre-treated with Fursultiamine (10 µM) for 30 minutes, followed by Sorafenib (5 µM) treatment for 24 h. Cells were harvested for trypan blue exclusion assay (A) or western blot assays with the antibodies as indicated (B, C). Actin blots served as the protein loading control. The asterisk indicates a significant difference compared to the DMSO control (p < 0.05, Student t-test).