Table 10.

Results of EZ-SARS-CoV-2 RT-PCR with pool size of 5 on the QuantStudio 5 platform for validation

Pool	Specimen	Individual Ct value	Pooled Ct valuea	Ct change
1	NP	32.96	35.04	2.08
2	NP	35.42	36.96	1.53
3	NP	32.65	35.04	2.39
4	NP	34.67	36.62	1.95
5	NP	33.11	36.97	3.86
6	NP	33.41	35.46	2.05
7	NP	34.74	36.84	2.11
8	NP	37.15	38.41	1.26
9	NP	35.13	37.04	1.91
10	NP	35.72	38.29	2.57
Average (standard deviation) of Ct change				2.17 (0.70)
11	NP	33.00	33.88	0.88
12	NP	31.60	31.67	0.08
13	NP	35.53	32.24	− 3.29
14	NP	34.79	36.05	1.26
15	NP	34.12	36.24	2.12
16	NP	32.28	33.53	1.25
17	NP	32.24	31.48	− 0.76
18	NP	28.76	30.35	1.59
19	NP	30.87	33.00	2.12
20	NP	32.36	34.82	2.46
Average (standard deviation) of Ct change				0.77 (1.73)
21	ANb	19.02	17.98	− 1.04
22	AN	19.86	18.73	− 1.13
23	AN	28.50	26.97	− 1.53
24	AN	31.64	38.44	6.80
25	AN	31.75	35.01	3.26
26	AN	32.96	37.95	4.99
27	AN	33.33	Not detected
28	AN	25.50	29.92	4.42
29	AN	34.00	36.82	2.82
30	AN	22.00	24.89	2.89
31	AN	23.75	25.98	2.23
32	AN	32.25	34.53	2.28
Average (standard deviation) of Ct change:				2.36 (2.67)

^aAverage Ct value from three independent extraction and RT-PCR runs

^bAN specimens and individual Ct values were kindly provided by the Wadsworth Center, NYS Department of Health. There was not enough volume for both individual and pooled extractions.