Table 2.

Final limit of detection (LoD) of EZ-SARS-CoV-2 Real-Time RT-PCR based on the analysis of twenty replicates at 250 copies per ml in AN matrix on the ABI 7500 platform

Replicate	Ct value	Replicate	Ct value	Replicate	Ct value	Replicate	Ct value
1	35.27	6	35.16	11	36.69	16	36.20
2	36.20	7	35.48	12	Undetermined	17	37.29
3	34.15	8	34.90	13	36.09	18	35.57
4	34.54	9	35.13	14	35.71	19	37.60
5	36.92	10	35.30	15	34.14	20	35.94