Table 3.

Intra-assay variation of EZ-SARS-CoV-2 real-time RT-PCR cycle threshold (Ct) values

Plate	Copies per ml	Mean (Ct)	SD (Ct)	% CV (Ct)	% CV (2−Ct)
ABI 7500 platform
1	104	29.91	0.09	0.30	6.18
1	103	33.56	0.05	0.16	3.78
1	102	36.74	0.72	1.96	53.52
2	104	29.86	0.03	0.10	2.09
2	103	33.92	0.26	0.76	16.96
2	102	38.07	0.83	2.19	62.83
3	104	29.45	0.12	0.41	8.47
3	103	32.94	0.37	1.14	26.08
3	102	35.78	0.54	1.5	36.76
QuantStudio 5 platform
1	104	29.99	0.39	1.30	26.64
1	103	33.02	0.08	0.24	5.67
1	102	36.49	1.14	3.14	84.4
2	104	29.88	0.28	0.94	19.55
2	103	34.13	0.32	0.93	22.28
2	102	36.13	0.49	1.36	31.15
3	104	29.99	0.15	0.51	10.37
3	103	33.28	0.13	0.38	8.60
3	102	37.05	0.45	1.21	30.91