Table 5.
Comparison of EZ-SARS-CoV-2 RT-PCR assay (EZ) results with TaqPath™ COVID-19 Combo Kit Multiplex Real-Time RT-PCR results for 201 NP samples performed on the same elution in the Cornell COVID-19 Testing Laboratory
| ABI 7500 | TaqPath positive | TaqPath negative | QuantStudio 5 | TaqPath positive | TaqPath negative |
|---|---|---|---|---|---|
| EZ positive | 92 | 10 | EZ positive | 91 | 9 |
| EZ negative | 0 | 99 | EZ negative | 2 | 99 |
| Diagnostic sensitivity | 100.00 (100.00-100.00) | Diagnostic sensitivity | 97.85 (94.90–100.80) | ||
| Diagnostic specificity | 90.83 (85.41–96.24) | Diagnostic specificity | 91.67 (86.45–96.88) | ||
| Positive predictive value | 90.20 (84.43–95.97) | Positive predictive value | 91.00 (85.39–96.61) | ||
| Negative predictive value | 100.00 (100.00–100.00) | Negative predictive value | 98.02 (95.30–100.74) | ||
| Kappa | 0.94 (0.90–0.97) | Kappa | 0.93 (0.89–0.97) | ||
Results determined on the ABI 7500 platform are on the left and results determined by the QuantStudio 5 platform are on the right. Estimates are followed by 95% confidence interval in parentheses