Table 9.

Results of EZ-SARS-CoV-2 RT-PCR with pool size of 5 on the ABI 7500 platform

Pool	Specimen	Individual Ct value	Pooled Ct valuea	Ct change
1	NP	33.55	34.58	1.03
2	NP	35.30	36.86	1.55
3	NP	33.39	34.34	0.95
4	NP	36.20	36.77	0.58
5	NP	33.85	36.55	2.71
6	NP	33.33	34.69	1.36
7	NP	35.75	36.62	0.87
8	NP	40.25	Not detected
9	NP	35.19	37.75	2.56
10	NP	35.93	37.51	1.58
Average (standard deviation) of Ct change				1.47 (0.74)
11	NP	30.83	33.94	3.10
12	NP	28.97	31.79	2.81
13	NP	33.41	32.40	− 1.01
14	NP	32.86	36.19	3.33
15	NP	33.95	36.49	2.54
16	NP	31.77	33.50	1.72
17	NP	32.00	31.16	− 0.85
18	NP	28.24	30.28	2.03
19	NP	30.16	33.28	3.11
20	NP	32.25	35.17	2.91
Average (standard deviation) of Ct change				1.97 (1.61)
21	ANb	19.02	18.15	− 0.87
22	AN	19.86	18.90	− 0.96
23	AN	28.50	27.09	− 1.41
24	AN	31.64	38.62	6.98
25	AN	31.75	34.71	2.96
26	AN	32.96	37.70	4.74
27	AN	33.33	37.22	3.89
28	AN	25.50	29.87	4.37
29	AN	34.00	39.12	5.12
30	AN	22.00	24.93	2.93
31	AN	23.75	25.93	2.18
32	AN	32.25	34.76	2.51
Average (standard deviation) of Ct change				2.70 (2.63)

^aAverage Ct value from three independent extraction and RT-PCR runs

^bAN specimens and individual Ct values were kindly provided by the Wadsworth Center, NYS Department of Health. There was not enough volume for both individual and pooled extractions