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Overexpression of mutant MetRS in MSCs allows tagging of nascent proteins in the cells in vitro and in vivo. (A) FACS analysis of mutant MSCs by the detection of mCherry signal. (B) Mutant or control cells were treated with or without ANL followed by FUNCAT analysis. Scale bar, 20 μm. (C) Gastrocnemius muscles in HLI and Sham group transplanted with mutant cells or PBS were collected and processed for FUNCAT analysis. Scale bar, 50 μm.
