FIGURE 4.

Identification of MSC-specific proteome in the hindlimb. The mice were subjected to HLI or Sham surgery, and mutant cells were i.m. injected in the gastrocnemius muscle. Mice were then randomly grouped and received i.p. injections of ANL at days 0, 2, 6, and 13, gastrocnemius muscles were collect 24 h later for protein enrichment and biological analysis. n = 3 mice for each time point in each group. (A) Nearly 200 proteins were identified in the eight groups. (B) Hierarchical clustering of proteins expressed in all groups. Each column corresponds to the mean expression levels of proteins in 3 independent biological samples, and each row corresponds to a protein.