Fig. 3.

The time course of the insulin effect on ERK1/2 and Akt phosphorylation. Cells were exposed to 100 nM insulin up to 5 min, homogenized and subjected to Western blot analysis for PERK1/2 and total ERK1/2 (upper part) and pAkt and total Akt (lower part). Increased band density signifies ERK1/2 or Akt activation. Each part shows a representative Western blot on the left and on the right the mean ± SEM of phospho vs total band density ratios calculated from three independent experiments. **(P < 0.01) and ***(P < 0.001) indicate significant differences from control.