Fig. 4.

Inhibition of SFK and p38 activation responses to insulin. Cells were pretreated with an SFK inhibitor PP2(10 μM) for 40 min or a p38 inhibitor SB202190 (2 μM) for 20 min and then were exposed to insulin (100 nM) for 2 min before Western blot analysis for pSFK 416 and β actin (upper part) and phospho p38 MAPK and total p38 (lower part). Some cells received insulin alone and control cells received neither insulin nor inhibitor. Each part shows a representative Western blot on the left and on the right the mean ± SEM of band density from three independent experiments. *(P < 0.05), **(P < 0.01), and ***(p < 0.001) indicate significant differences from control. - (P < 0.01) indicates a significant difference from insulin treatment.