Fig. 9.

The influence of ERK1/2 inhibitor U0126, SFK inhibitor PP2 and p38 inhibitor SB202I90, on the insulin-induced increase in Na,K-ATPase activity. Na,K-ATPase activity (ouabain-sensitive ATP hydrolysis rate) was measured in homogenates prepared from cells pretreated with U0126 (10 μM) (upper left part) or SB202190 (2 μM) (lower part) for 20 min or with PP2 (upper right part) for 40 min then exposed to 100 nM insulin for 5 min in the continued presence of the respective inhibitor. Some cells were exposed to insulin alone and control cells received neither insulin nor inhibitor. Values shown are the mean ± SEM of results from four independent experiments. **(P < 0.01) and ***(p < 0.001) indicate significant differences from control. -(P < 0.01) indicates a significant difference from insulin treatment.