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. 2021 Jun 30;12:668897. doi: 10.3389/fpls.2021.668897

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Copyright © 2021 Favier, Gans, Boeri Erba, Signor, Muthukumar, Pfannschmidt, Blanvillain and Cobessi.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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PAP9 is localized in plastids. (A) Schematic illustration of the PAP9-GFP construction in pAF04. p35S, CaMV35S promoter region; cTP, chloroplast transit peptide in yellow; ?, predicted NLS (nuclear localization signal) in red; C-terminal domain in magenta; GFP in green. (B) Transiently expressed PAP9-GFP in onion epidermal cells. N, nucleus; str, stromule; p, plastid. The red arrowhead points to the absence of red fluorescence in stromules. The yellow rectangle represents the analyzed segment in panel (C). (C) Fluorescent signal quantitative profile on an 18-μm-long segment of the image across three plastids. Δ represents the difference in width of the GFP signal compared to the red signal of PAP10-RFP.