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Demonstration of thermogram baseline subtraction (ITC power trace obtained in 0.3 mM MgCl2 at 30 °C, using starting cell [RR] = 10 µM, syringe [TT] = 171 µM, and 6.5 µL injections.) A) Raw Thermogram (black dots) and fitted baseline (gray curve). B) Following baseline subtraction, the plotted data express only the additional power input in response to ligand injection and binding (referred to here as EXS).
