Figure 2.
Demonstration of injection peak fit preparation procedure, using the 7th injection from the titration in Figure 2. A) Deconvolution to recover the instantaneous power signal. The black (raw) data points are referred to as EXS (the instrument power response) and the green triangles (post-deconvolution) are equivalent to E* (the instantaneous rate of heat evolution due to injection and macromolecular association). B) Baseline, fitting regime, and curve fitting. A final baseline was not fully established in this injection, so the blue dotted line represents the linear and constant terms determined from a preliminary regression procedure. Data within the fitting region are highlighted by large blue circles. The starting point for fitting, t0, corresponds to the minimum in the pre-deconvolution data (44 seconds) and, in this case, the final point, tf, is determined from the decay in the power signal (504 seconds). The red curve represents the results of regression using the general set of kinetics equations (2, 5 and 6).