Table 2.
Concentration-Dependent Near-Equilibrium Test for TT – RR experiments in 0.3 mM MgCl2, 30 °C
| Dataset(s) | Kd (nM) | Near-Equilibrium Approximation | General Method | ||||||
|---|---|---|---|---|---|---|---|---|---|
| kon (M−1s−1) | koff (s−1) × 10−5 | kon (M−1s−1) | kon·Kd (s−1) × 10−5 | ||||||
| [M]init = 5 µM RR | 900 (± 400) | 930 | {± 580} [± 630] (± 640) |
30 | {± 220} [± 240] (± 250) |
790 | {± 40} [± 190] (± 190) |
71 | {± 32} [± 36] (± 36) |
| [M]init = 10 µM RR | 910 (± 80) | 730 | {± 110} [± 180] (± 180) |
160 | {± 80} [± 110] (± 110) |
790 | {± 10} [± 150] (± 150) |
72 | {± 6} [± 15] (± 15) |
| [M]init = 20 µM RR | 1300 (± 200) | 940 | {± 50} [± 170] (± 170) |
−110 | {± 60} [± 160] (± 160) |
750 | {± 10} [± 120] (± 120) |
99 | {± 10} [± 19] (± 19) |
| avg (plotted) | 1000 (± 200) | 840 | (± 140) | 68 | (± 89) | 770 | (± 80) | 84 | (± 52) |
| avg (all) | 2000 (± 100) | 710 | (± 90) | 15 | (± 65) | 470 | (± 30) | 96 | (± 7) |
{} includes all injection-level uncertainty terms
[] includes all injection-level and experiment-level uncertainty
() includes all uncertainty contributions