Skip to main content
. Author manuscript; available in PMC: 2021 Jul 14.
Published in final edited form as: Methods Enzymol. 2015 Oct 9;567:181–213. doi: 10.1016/bs.mie.2015.08.012

Table 3.

Concentration-Dependent Near-Equilibrium Test for TT-RR experiments in 200 mM KCl, 25 °C

Dataset(s) Kd (nM) Near-Equilibrium Approximation General Method
kon (M−1s−1) koff (s−1) × 10−5 kon (M−1s−1) kon·Kd (s−1) × 10−5
[M]init = 4 µM RR 250 (± 60) 7300 {± 700}
[± 1700]
(± 1800)		 280 {± 140}
[± 360]
(± 360)		 7300 {± 100}
[± 1200]
(± 1200)		 180 {± 50}
[± 60]
(± 60)
[M]init = 8 µM RR 300 (± 20) 8100 {± 500}
[± 1400]
(± 1900)		 –10 {± 210}
[± 460]
(± 560)		 7000 {± 100}
[± 1000]
(± 1100)		 210 {± 10}
[± 30]
(± 40)
[M]init = 16 µM RR 400 (± 20) 7400 {± 300}
[± 1100]
(± 2300)		 –110 {± 260}
[± 580]
(± 960)		 6800 {± 100}
[± 700]
(± 1500)		 270 {± 10}
[± 30]
(± 60)
avg (plotted) 360 (± 10) 7600 (± 1500) 100 (± 450) 6900 (± 900) 250 (± 30)

{} includes all injection-level uncertainty terms

[] includes all injection-level and experiment-level uncertainty

() includes all uncertainty contributions