Fig. 2. Application of M2 MspA in DNA sequencing. The schematic diagram (left) and a representative trace of nanopore sequencing of DNA (right). Nanopore sequencing was performed as previously published.⁹ The measurements were performed in a 0.3 M KCl buffer (0.3 M KCl, 10 mM MgCl₂, 10 mM (NH₄)₂SO₄, 4 mM DTT and 10 mM HEPES, pH = 7.5) buffer. A +180 mV potential was continuously applied. A DNA template, primer and blocker (Table S1†) were designed for a simple demonstration of nanopore sequencing. Phi29 DNA polymerase (green, left diagram) was applied as the molecular motor during nanopore sequencing. M2 MspA (grey, left diagram) applied for these measurements was from the corresponding sample as demonstrated in the gel results of Fig. 1.