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. Author manuscript; available in PMC: 2022 Mar 1.
Published in final edited form as: Mech Ageing Dev. 2020 Dec 28;194:111425. doi: 10.1016/j.mad.2020.111425

Figure 1: Follicular density decreases with age.

Figure 1:

Box plots demonstrates age-related decreases in follicular density represented as the mean of 5 ovarian sections per mouse (n = 5 mice/ group; *p<0.05, **p<0.01, ***p<0.005 and ****p<0.001; one-way ANOVA with Tukey post hoc test). For each section the follicular density was calculated as the number of follicles in the section divided by the area of the section. (A) Total, all follicles with clearly visible oocyte nucleus (B) Primordial follicles representing follicles surrounded by a single layer of flattened granulosa cells (C) Transition + Primary representing follicles surrounded by single layer of cuboidal cells or both flattened and cuboidal cells (D) Secondary follicles are surrounded by more than one layer of granulosa cuboidal cells with no visible antrum (E) Tertiary represents follicles with well-defined antral space and a layer of cumulus granulosa cells around the oocyte.