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. Author manuscript; available in PMC: 2021 Jul 14.
Published in final edited form as: Brain Behav Immun. 2020 Sep 19;91:578–586. doi: 10.1016/j.bbi.2020.09.014

Fig. 1. B- and T-Lymphocyte infiltration into the infarct following stroke is delayed.

Fig. 1.

A.) Representative images of anti-B220 immunostaining of brains sections from naïve mice and mice 24 hours, 1 week, 2 weeks, 4 weeks, and 7 weeks following DH stroke. Images are taken at 10x and insets are 40x. Scale bar, 500 μm 10x and 150 μm 40x. B.) Percent area stained by B220 within the stroke infarct at 24 hours, 1 week, 2 weeks, 4 weeks, and 7 weeks following DH stroke. B-lymphocytes first appear within the infarct at 2 weeks post-stroke, and their numbers are significantly increased at 4 weeks and 7 weeks. C.) Representative images of anti-CD3ε immunostaining of brains sections from naïve mice and mice 24 hours, 1 week, 2 weeks, 4 weeks, and 7 weeks following DH stroke. Images are taken at 10x and insets are 40x. Scale bar, 500 μm 10x and 150 μm 40x. D.) Percent area stained by CD3ε within the stroke infarct at 24 hours, 1 week, 2 weeks, 4 weeks, and 7 weeks following DH stroke. T-lymphocytes are significantly increased at 2 weeks, 4 weeks, and 7 weeks following stroke. n = 5–7 per group. Data represent mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 by one-way ANOVA with comparisons against all other groups, with post-hoc Tukey’s multiple comparisons test.