Figure 7.

cDC1, type I IFN and type II IFN signalling are necessary for CD4⁺ and CD8⁺ T‐cell terminal activation and effector functions in the TME. (a) Kinetics of the tumor infiltration by Ag‐specific CD4⁺ (up) and CD8⁺ (bottom) T cells at d4, d7 and d15 in Ifnar1 ^−/−, Ifngr1 ^−/− Xcr1‐DTA and control mice. The data shown (mean ± SEM) are from two independent experiments pooled together (n = 3–6 mice per group). (b) Heatmap representing T‐cell effector phenotype and proliferation, and CTL cytokine production. The data are shown as log₂ fold changes in the ratio of % Population/Parent population from mutant animals to WT (n = 3–6 mice per group). (c) Expression of GzmB, IFN‐γ and TNF by OVA‐specific CD44⁺ CD8⁺ T cells in the TdLN of Ifnar1 ^−/−, Ifngr1 ^−/−, Xcr1‐DTA and control mice day 7 post‐engraftment. The data shown are from two independent experiments pooled together. For a and c, data are shown as mean ± SEM, with values for individual mice shown as white circles in (c). *, P < 0.05; **, P < 0.01; and ***, P < 0.001; non‐parametric Mann–Whitney U‐test.