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. 2021 Jul 1;17(7):e1009475. doi: 10.1371/journal.pgen.1009475

Fig 5. CATP-8/P5A-ATPase can function cell autonomously and non-autonomously during neural patterning.

Fig 5

A.—B. Maximum z-projection of confocal images of the PVD::GFP marker wdIs52 from non-transgenic (A) and transgenic (B) sibling animals carrying the pan-neuronal catp-8 rescueing array in a catp-8(gk860114) mutant background, showing restoration of PVD structure upon neuronal expression. Scale bar = 20μm. C.—D. Maximum z-projection of confocal images of the PVD::GFP marker wdIs52 from animals without (C) and with (D) single-copy insertion of epidermal catp-8 rescue under catp-8 mutant background, showing restoration of PVD structure under epidermal rescue. Scale bar = 20μm. E.—G. Quantification of number of quaternary branches (E), secondary branches (F), and the ratio of quaternary to secondary branches (G) 100 μm anterior to the PVD cell body in animals of genotypes indicated, showing complete rescue of catp-8(gk860114) phenotype when catp-8 is expressed from pan-neuronal or epidermal promoter, but not muscle promoter. TG: transgenic; NTS: non-transgenic siblings. Data are represented as the mean ± 95% confidence interval. ****, P<0.0001, ns, not significant, Kruskal-Wallis test with Dunn’s multiple comparisons test. n = 14 animals per genotype. H.—J. Percentage of animals with the indicated migration phenotype of HSN (H), PQR (I) and PVM (J) in the indicated genotypes, showing that all migration phenotypes of catp-8 were rescued completely when catp-8 was expressed from muscle, but not when expressed cell-autonomously, pan-neuronally or epidermally. **** P < 0.0001, ns not significant, Chi-squared test. Sample size indicated on graph.