Table 1. Survey of neuroanatomical defects in catp-8/P5A-ATPase mutant animals.
Neurons Examined (Marker Used) | control [%] | N | catp-8 [%] | N | P Value1 |
---|---|---|---|---|---|
Motor Neurons2 | |||||
D-Type (juIs76) | |||||
Gaps in ventral nerve cord | 10.8 | 93 | 41.3 | 109 | **** |
Gaps in dorsal nerve cord | 12.9 | 93 | 26.6 | 109 | * |
0 L/R choice commissure | 60.0 | 40 | 23.6 | 55 | *** |
1 L/R choice commissure | 25.0 | 40 | 41.8 | 55 | ** |
≥ 2 L/R choice commissure | 15.0 | 40 | 32.7 | 55 | |
defasciculation of DNC | 0.0 | 31 | 11.4 | 44 | 0.07 |
defasciculation of VNC | 19.4 | 31 | 36.4 | 44 | 0.13 |
defasciculation in both | 3.2 | 31 | 2.3 | 44 | ns |
DA/DB (evIs82b) | |||||
Gaps in ventral nerve cord | 0 | 102 | 0 | 108 | ns |
Gaps in dorsal nerve cord | 0 | 102 | 0 | 108 | ns |
L/R choice of commissures | 4.8 | 102 | 5.7 | 108 | ns |
HSN (mgIs71) | |||||
Ventral nerve cord crossover2 | 0.0 | 85 | 12.5 | 72 | *** |
Interneurons3 | |||||
PVQ (oyIs14) | |||||
Ventral cord crossover | 11.8 | 101 | 7 | 100 | ns |
AIY (mgIs32) | |||||
Early termination | 0 | 120 | 0 | 124 | ns |
Ectopic axonal branching | 2 | 120 | 2 | 124 | ns |
Amphid Neurons4 | |||||
DiI Stain | |||||
Defasciculation | 6 | 50 | 4 | 50 | ns |
Early termination | 0 | 50 | 0 | 50 | ns |
Anterior process | 0 | 50 | 0 | 50 | ns |
Anterior nerve ring | 0 | 50 | 0 | 50 | ns |
Posterior process | 0 | 50 | 0 | 50 | ns |
Posterior nerve ring | 0 | 50 | 0 | 50 | ns |
Phasmid Neurons3 | |||||
DiI Stain | |||||
Ectopic axons | 0 | 50 | 0 | 50 | ns |
Axon crossover | 0 | 50 | 0 | 50 | ns |
Wandering processes | 0 | 50 | 0 | 50 | ns |
1 Statistical significance was calculated using Fisher’s exact test.
*, P ≤ 0.05
**, P ≤ 0.005
****, P ≤ 0.0001; ns, not significant.
2 Note that the cross over defects invariably occurred when HSNL failed to migrate, in which case a process first grew out posteriorly, before turning ventrally. The process then crossed the midline and grew anteriorly in the right ventral nerve cord.
3 Neuroanatomical defects were scored as described [65].
4 Neuro-anatomical defects were scored as described [66].