Figure 9.

CD4⁺ and CD8⁺ T cell activation, proliferation, and cytokine secretion in response to ovalbumin presentation by bone-marrow-derived dendritic cells (BMDCs) pretreated with desmuramylpeptides 68 and 75. BMDCs from C57BL/6 mice were treated with 68 and 75 (1 and 10 μM) and ovalbumin (50 μg/mL). After 18 h, they were washed and cocultured for 72 h with carboxyfluorescein succinimidyl ester (CFSE)-labeled ovalbumin-specific CD4⁺ or CD8⁺ T cells, isolated from OT II or OT I mouse splenocytes, respectively. (A) Representative dot plots of live Thy1.2⁺/CD4⁺ or Thy1.2⁺/CD8⁺ T cells, showing CD25 expression and CFSE dilution. (B) Quantification of the proportions of CD25⁺, CFSE^low T cells. The analysis regions are shown in panel A. (C) Cytokine concentrations in coculture supernatants following co-incubation with BMDCs as described in panel A. Data are expressed as mean ± SEM of duplicates of two independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001.