. 2021 Jul 14;15(7):e0009517. doi: 10.1371/journal.pntd.0009517

Table 1. Primer sequences for Leishmania and Bartonella DNA amplification from sand flies.

Locus	Primers		Primer 5’-sequence-3’	Size of PCR Product (bp)	PCR Protocol Reference
*Bartonella*
16S–23S internal transcribed spacer	ITS	325S	CTTCAGATGATGATCCCAAGCCTTTTGGCG	400–600	[51]
16S–23S internal transcribed spacer	ITS	1100AS	GAACCGACGACCCCCTGCTTGCAAAGCA	400–600	[51]
Citrate synthase gene	gltA	443F	GCTATGTCTGCATTCTATCA	767	[11]
		1210R	GATCYTCAATCATTTCTTTCCA	767	[11]
		BhCS.781p	GGGGACCAGCTCATGGTGG	380–400	[52]
		BhCS.1137n	AAATGCAAAAAGAACAGTAAACA	380–400	[52]
Filamenting temperature-sensitive mutant Z protein gene	ftsZ	BFP-1	ATTAATTCTGCAYCGGCCAGA	600	[58]
		BFP-2	ACVGADACACGAATAACACC		[58]
		R83	ATATCGCGGAATTGAAGCC		[59]
		L83	CGCATAGAAGTATCATCCC		[59]
RNA polymerase β subunit gene	rpoB	1350F	GGCAATCGTCGCGTTCGTTC	852	[56]
		2350R	CTACCCGATCACCAACATGC		[56]
		1400F	CGCATTGGCTTACTTCGTATG		[57]
		2300R	GTAGACTGATTAGAACGCTG		[57]
*Leishmania*
kinetoplast DNA	L.MC	L.MC-1S	CTRGGGGTTGGTGTAAAATAG	700	[48]
kinetoplast DNA	L.MC	L.MC-1R	TWTGAACGGGRTTTCTG	700	[48]
cytochrome b gene	L.cyt-B	L.cyt-AS	GCGGAGAGRARGAAAAGGC	1070–1080	[49]
		L.cyt-ASR	CCACTCATAAATATACTATA	1070–1080	[49]
		L.cyt-S	GGTGTAGGTTTTAGTYTAGG	730–850	[48]
		L.cyt-R	CTACAATAAACAAATCATAATATRCAATT	730–850	[48]