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. Author manuscript; available in PMC: 2022 Jan 1.
Published in final edited form as: Dev Biol. 2020 Sep 25;469:12–25. doi: 10.1016/j.ydbio.2020.09.014

Figure 2: Knock down of Diaphanous impairs thin filament growth in IFMs.

Figure 2:

A-B. Changes in IFM sarcomere size in wild-type adult flies. Wild-type flies were dissected at days 1 (newly eclosed), 3, and 7 after eclosion. The length and width of the individual sarcomeres was measured and recorded (5 flies per genotype, n = 5 average sarcomere length or width measurements/fly). Sarcomeres in newly eclosed flies are significantly shorter (p<0.0001) and thinner (p<0.0001) than the sarcomeres of flies 3 days after eclosion. There is no significant difference in sarcomere size between day 3 and day 7 flies. These data are consistent with continued growth of the sarcomeres in newly eclosed flies (day 1), followed by sarcomere maintenance (thereafter). Scale bar: 5μm C-D. Sarcomere phenotypes upon Dia knockdown in the muscle. Sarcomere size was measured on Day 1, 3 and 7 after eclosion (5 flies per genotype, n = 5 average sarcomere length or width measurements/fly). There is no significant difference in sarcomere size between each group. E. Diagram of experimental design. dia-RNAi was induced in adult muscle 3 days after eclosion. F. Viability of adult fly was examined after induction of dia-RNAi. No significant difference in viability was observed between control (n = 56 flies) and Dia knockdown (n = 52 flies) groups at the indicated time points. G. Quantification of Flight Performance following Dia knockdown. Flight performance was measured after 0, 4 and 11 days of induced dia-RNAi (3, 7, and 14 days after eclosion). Difference of flight performance was observed between control (Day 0; n = 140 flies, Day 4; n =51 flies, Day 7;n = 103 flies) and Dia knockdown (Day 0; n = 67 flies, Day 4; n = 54 flies, Day 7;n = 85 flies) groups following 4 (p<0.05) and 11 (p<0.0001) days of induction. H-J. Sarcomere morphology and size was examined after dia-RNAi induction. Average myofibril measurements were made at the indicated days of knockdown induction. Four days after induced Dia knockdown, sarcomere length and width are significantly reduced compare to control (5 flies per genotype, n = 5 average sarcomere length or width measurements/fly, ****p<0.0001). Scale bar: 2μm.