Fig. 5.

MST4 regulates oxidative and ER stress in IHHs. IHHs were transfected with MST4 siRNA or NTC siRNA (A), or with MYC‐tagged MST4 expression plasmid or an empty control plasmid (B), and challenged with OA for 48 hours. Representative images of cells stained with DHE (red) or processed for immunofluorescence with anti‐8‐oxoG (red), anti‐4‐HNE (green), anti‐E06 (green), anti‐KDEL (green), or anti‐CHOP (red) antibodies; nuclei stained with DAPI (blue). Quantification of the staining. The scale bars represent 25 µm. Data are presented as mean ± SEM from eight wells per group. *P < 0.05, **P < 0.01, ***P < 0.001.