Figure 11.

Effects of (R)-6 on intracellular calcium flux in stably transfected HEK-293. Cells were cultured for 18 h prior to labeling with Fluo-4 (37 °C, 1 h). (a) Quantification of three independent experiments was carried out by calculating differential calcium signals measured at the baseline and at maximum peak. Data are expressed as % delta calcium-induced fluorescent signal relative to the peak of a known full agonist (1) ± SEM (n = 3). Concentration–response curves for LXA₄ (1) and sLXms (10 pM to 100 nM) show the E_max (black circles) and predicted EC₅₀ (white dotted circles). Statistical analyses were carried out using Student’s unpaired t-test of the tested compound vs veh (not shown).