Figure 3.
Effect of series (6) of QNX-sLXms on LPS-induced NF-κB-driven luciferase activity in monocytes. A total of 1 × 105 THP-1 LUCIA monocytes were pretreated for 30 min with sLXms, vehicle, or appropriate controls, at indicated concentrations in the presence (LPS-activated) or absence (basal) of 50 ng/mL LPS. After 24 h, supernatants were collected and NF-κB-driven luciferase activity was assayed. (a) Single-point analysis of the internal controls. (b) Concentration–response curves of reference compounds (1, 5) and QNX-sLXms. Data are expressed as % ± SEM (n = 3) of normalized luminescence unit relative to LPS-induced response. Best-fitting curves are indicated by black solid lines. Statistical analysis was carried out using Student’s unpaired two-tailed t-test of the tested compound vs LPS (*p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001) or vs LXA41 (not shown).