Figure 4.
Effect of QNX-sLXm lead compound (R)-6 on the whole panel of proinflammatory cytokine release in monocytes. A total of 1 × 105 THP-1 LUCIA monocytes were pretreated for 30 min at indicated concentrations with LXA41 (i) or QNX-sLXm (R)-6 (ii). After 24 h from the subsequent stimulation with LPS, supernatants were collected and a panel of seven proinflammatory cytokine levels was measured (IL-6, IL-1β, IFN-γ, IL-12p70, IL-8, IL-10, TNF-α). Concentration–response curves show upregulated (blue), downregulated (red), or unmodified (black) cytokines. Statistical analysis was carried out using Student’s unpaired two-tailed t-test of the tested compound vs LPS (ns, not significant; ****p < 0.0001).