Figure 6.
Extrinsic cytotoxic profile of series (6) of QNX-sLXms. A total of 1 × 105 THP-1 LUCIA monocytes were pretreated for 30 min with QNX-sLXms, vehicle, or appropriate controls (1 pM–1 μM) and subsequently challenged for 24 h with 50 ng/mL LPS. After 24 h, supernatants were collected and LDH release was assayed. (a) Single-point analysis of controls defines a high (red area), moderate (yellow area), and low (gray area) level of cytotoxicity. (b) Concentration–response and best-fitting curves of (R)-6 and (S)-6. Data are expressed as % LDH release relative to LPS ± SEM (n = 3). Statistical analysis was carried out using Student’s unpaired two-tailed t-test of the tested compound vs LPS (*p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001).