Skip to main content
. 2021 Jul 15;131(14):e146893. doi: 10.1172/JCI146893

Figure 6. The AAA ATPase of TRIP13 is required for USP7 deubiquitinase function.

Figure 6

(A) TRIP13 IP of ARP1 TRIP13-OE cells treated with oligomycin A for 6 hours followed by Western blot analysis of FT, LW, and E for TRIP13 and USP7. (B) TRIP13 IP from HEK293T cells transduced with FLAG-USP7 or FLAG-USP7 and WT HA-TRIP13, truncation 1 (Δ1) HA-TRIP13, or truncation 2 (Δ2) HA-TRIP13 followed by Western blot analysis of FLAG or HA. (C) Deubiquitination time course and Western blot analysis of Ub derived from recombinant K63-Ub in the presence of recombinant USP7 with or without purified TRIP13-WT or TRIP13-E253Q mutant (“253”).