FIGURE 3.

Podocyte OST48 was associated with a deterioration of podocyte architecture. (A‐C) Assessment of renal glomerular damage in the kidney with (A) Periodic acid Schiff staining (PAS) and (B) collagen IV, which was then quantified based on a positive threshold protocol. DDOST+/−Pod‐Cre mice and mice with a diabetic phenotype show moderate glomerulosclerosis, indicated by an increase in mesangial matrix expansion (black arrow) and accumulation of the extracellular matrix (white arrow head). (C) Electron microscopy determined the degree of hypertrophy in the foot processes (black arrow) and the expansion of the GBM (white arrow head). Scale bars from representative images of (A‐B) glomeruli stained with either PAS or collagen IV were 20 μm. (C) Representative images from electron microscopy were imaged at either × 3000 magnification or × 15000 magnification were 20 μm and 4 μm, respectively. (D) Heat map representation of SWATH‐MS proteomics data for enzymatic pathways involved in collagen fibril organization. Significant proteins are represented as bolded cells, where red indicates an increase and blue indicates a decrease in protein concentrations. Data represented as means ± SD (n = 4‐7/group). Results are expressed as mean ±SD with either two‐way ANOVA or unpaired t‐test analysis (n = 5–9) *p < .05, **p < .01, ***p < .001, ****p < .0001. For proteomics, MSstatsV2.6.4 determined significant (p < .05) log fold changes in the protein intensities between the selected experimental group the wild‐type nondiabetic group. Heatmap representation allow for compact visualization of complex data comparisons. Full detail of the quantitative data is available in the supplementary information