FIGURE 4.

Podocyte OST48 increased tubulointerstitial fibrosis. (A‐C) Moreover, the presence of collagen in (A) Masson's trichrome (blue staining), (B) Sirius red (red) and (C) collagen IV (brown) was quantified based on a positive threshold protocol. Fibrosis in the interstitium of the proximal tubules (black arrow) is an indicator of progressive kidney damage. The severity of these changes was more pronounced in the DDOST+/−Pod‐Cre mice and the mice with a diabetic phenotype. (D) Heat map representation of SWATH‐MS proteomics data for enzymatic pathways involved in collagen fibril organization. Significant proteins are represented as bolded cells, where red indicates an increase and blue indicates a decrease in protein concentrations. Data represented as means ± SD (n = 4‐7/group). Scale bars from representative images of (A‐C) proximal tubule sections stained with either Masson's trichrome, Sirius red or collagen IV were 100 μm. Results are expressed as mean ± SD with either two‐way ANOVA or unpaired t test analysis (n = 5–9) *p < .05, **p < .01. For proteomics, MSstatsV2.6.4 determined significant (p < .05) log fold changes in the protein intensities between the selected experimental group the wild‐type non‐diabetic group. Heatmap representation allow for compact visualization of complex data comparisons. Full detail of the quantitative data is available in the supplementary information