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. 2021 Jun 22;4(3):e00278. doi: 10.1002/edm2.278

FIGURE 5.

FIGURE 5

Podocyte OST48 increased AGE accumulation in podocytes. (A) Confocal photomicrographs of OST48 (green), CML (orange) and a podocyte foot process marker, nephrin (red) on kidney sections imaged at a glomerulus. (B) 3D‐SIM of podocyte foot processes staining for nephrin (red), OST48 (green) and CML (blue) localization. (C) 3D‐SIM reconstruction of podocyte foot processes staining for nephrin (red), OST48 (green) and CML (blue) localization. (D‐E) CML ELISA measuring the total content of CML detected in (D) whole kidney cortex homogenates and in (E) 24‐hour urine collections. Scale bars from representative images for (A) confocal microscopy and (B‐C) 3D‐SIM were 30 μm and 5 μm, respectively. Results are expressed as mean ± SD with either two‐way ANOVA or unpaired t test analysis (n = 5–9) *p < .05