FIGURE 6.

Podocyte OST48 increased ER stress markers. (A) Confocal photomicrographs of either ER stress marker GRP‐78 (green), or XBP‐1 (green) and podocyte foot process marker, synaptopodin (red). (B‐C) Heat map representation of SWATH‐MS proteomics data for enzymatic pathways involved in (B) endoplasmic reticulum (ER) stress and oxidative stress (OS) enzymatic pathways, and (C) ubiquinone biosynthesis pathways. Significant proteins are represented as bolded cells, where red indicates an increase and blue indicates a decrease in protein concentrations. Data represented as means ±SD (n = 4‐7/group). Scale bars from representative images of confocal microscopy were 30 μm. Data represented as means ±SD (n = 5/group). For proteomics, MSstatsV2.6.4 determined significant (p < .05) log fold changes in the protein intensities between the selected experimental group the wild‐type non‐diabetic group. Heatmap representation allow for compact visualization of complex data comparisons. Full detail of the quantitative data is available in the supplementary information