Figure 6.

Ouabain inhibited the protein synthesis of STAT3. (A, B) A549 and Hela cells were exposed to ouabain for 24 hours, and STAT3 mRNA expression was determined by PCR. (C, D) A549 and Hela cells were treated with vehicle or ouabain in the presence or absence of cycloheximide (CHX) (C) or MG132 (D), the expressions of STAT3 were analyzed by Western blotting. (E) A549, Hela and HCT116 cells were treated with different concentrations of ouabain for 24 hours, the expressions of p-mTOR, mTOR, p-4EBP1, 4EBP1 and eIF4E were analyzed by Western blotting. (F) Immunoprecipitation analysis of eIF4E and eIF4G in Hela cells treated with or without ouabain.