Skip to main content
. 2021 Jul 14;10:e67399. doi: 10.7554/eLife.67399

Figure 3. Expression of GFP-tagged ribosomes and ribosomal binding of transcripts in PCs.

Figure 3.

(a) Schematic of TRAP protocol for identifying ribosomally bound transcripts specifically within PCs. (b) Anti-GFP immunofluorescence of in Rosa26fsTRAP L7Cre animals shows labeling of the PCs of the cerebellum. Sagittal sections from P56 Rosa-Trap animals expressing Cre under Pcp2 promoter (L7Cre) were stained with GFP (green), calbindin (red), and DAPI (blue). Scale bars, 10 µm. (c) Heatmap of TRAP levels of genes that were previously identified to be downregulated at the transcript level. Each row of the heatmap represents the scaled expression of one transcript, where red corresponds to higher relative expression and blue corresponds to lower relative expression. (d) This histogram shows the fold changes of the TRAP values for the previously identified downregulated transcripts between mutant and control PCs. Despite being downregulated at the total mRNA level, these transcripts generally show maintained ribosomal binding.