TABLE 2.

Detection of C. botulinum type D/C in the poultry house after the cattle outbreak (n = 128) using swab samples.

		After extensive disinfection of the poultry house n = 9		Upon chick delivery n = 55			Upon broiler departure n = 64
Flock No 1*	21/03/18	Inside the house n = 7	Ventilation system (S5) 2 + /4 Floor of the house 0 + /1 Changing room 0 + /1 Feed silo 0 + /1
		Outside the house n = 2	Carcasses bin (S4) 1 + /1 Surroundings 0 + /1
Flock No 2*						ND			ND
Flock No 3*				22/05/18	Inside the house n = 7	Ventilation system 2 + /4 Floor of the house 0 + /1 Changing room 0 + /1 Feed silo 0 + /1	13/06/18	Inside the house n = 1	Floor 0 + /1
					Outside the house n = 2	Surroundings 0 + /1 Carcasses bin 0 + /1		Broilers n = 20	Cloacal swabs 4 + /20 (S6 and S7)
Flock No 4*				9/07/18	Inside the house n = 2	Ventilation system 0 + /2	6/08/18	Inside the house n = 3	Ventilation system 2 + /2 Floor 1 + /1
					Material from the hatchery n = 2	Chick box bottom 2 + /2		Broilers n = 20	Cloacal swabs 17 + /20 (S11 to S13)
Flock No 5*				27/08/18	Inside the house n = 2	Ventilation system 2 + /2	24/09/18	Broilers n = 20	Cloacal swabs 0 + /20
					Material from the hatchery n = 16	Chick box bottom 0 + /2 Article of the box bottom 0 + /10 10 chick boxes upon arrival 0 + /2 Trolley and its wheels upon arrival 1 + /2 (S8)
					Chicks from the hatchery n = 20	Cloacal swab on chicks 0 + /20
					Material returned to the hatchery n = 4	10 chick boxes upon departure 0 + /2 Trolleys and their wheels upon departure 0 + /2

ND, no data.

*Number of positive samples/number of samples collected (positive samples are in bold).

Samples tested by MLVA are underlined and the sample code inFigure 2 is indicated in parentheses.