TABLE 3.

Detection of C. botulinum type D/C at the hatchery delivering chicks on the farm (n = 58).

	Sampling location	Detection of C. botulinum type D/C
Inside the hatchery *n = 41	Egg receiving room Incubation room Transfer (to hatcher) room Hatcher room Changing room Chick sorting room Chick departure room	0 + /4 0 + /12 0 + /2 0 + /11 0 + /3 1 + /5 Swab of the machine cutting the article box containing the chicks 0 + /4
vOutside the hatchery * n = 9	Trucks platforms Waste containers Central area Breeder house surroundings	1 + /3Swab of the loading platform 1 + /3Swab of the container of sludge washing water 0 + /1 0 + /2
Annex room* n = 8	Wash room (uncleaned egg trolleys) Clean room (cleaned egg trolleys) Egg holding (cooler) room	1 + /3 Swab of the room of the uncleaned egg trolleys (S9) 2 + /3 Swab of the room of the cleaned egg trolleys and swab of the trolley wheels (S10) 0 + /2

*Number of positive samples/number of samples collected. Samples tested by MLVA are underlined and the sample code inFigure 2 is indicated in parentheses. Samples detected positive are in bold.