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Immunofluorescence labeling of MAP2 and NF-L at different time points in the mouse model of MCAO: the reduction of MAP2-related immunoreactivity (green) identifies the infarct border (dashed line) in the cortex of mice at all time points of transient and permanent ischemia. The NF-L-related immunoreactivity (red) is increased in the infarct area, with a clear increase after 24h and 72h, but no apparent increase after 4h-t. Scale bars: 200μm
