Fig. 4.

a, b Western blot analyses of MAP2 and NF-L in mice after 4 and 24 h of transient and permanent MCAO: MAP2 levels decrease in stroke tissue throughout the applied models, although without reaching significance. c–f) Western blot was performed with a polyclonal “pab” NF-L antibody (c, e, f) and a monoclonal antibody “mab” (d). Thereby a decrease of NF-L in infarcted tissue was measured, which was most pronounced after 24h-p, but failed to reach statistical significance. NF-L levels after 4h-t remained mostly unaltered. e, f NF-L degradation products of 40-65 kDa are significantly increased after 24h-p. n=5–6. Data are presented as mean values; error bars indicate the standard error. **p<0.01, ***p<0.001