Fig. 5.

a Electron microscopy images show cross sections of cortical axons after 4h and 24h of transient ischemia in mice and unaffected contralateral regions. For illustration, transversally sectioned axons are transparently highlighted in yellow. The regular distribution of axonal neurofilaments is lost (insets) 4h and 24h after tMCAO. An ischemia-induced cellular edema is indicated by a less electron dense cytoplasm in the highlighted axons and insets as well as in non-axonal structures (asterisks). Please note that the images and insets also contain other components of the axonal cytoplasm which were not considered for the analysis of neurofilament density. b The density of detectable neurofilaments is decreased in ischemia-affected cortical axons 4h and 24h after tMCAO. n=5. Data are presented as mean values; error bars indicate the standard error. *p<0.05. Scale bars: 1 μm