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. 2021 Jul 15;12(7):705. doi: 10.1038/s41419-021-03996-y

Fig. 1. Synergistic antitumor effect of DHA in combination with DDP.

Fig. 1

A, B Pancreatic cancer cells were subjected to different concentrations of DHA and DDP treatment for 24 h and cell viability was detected by CCK-8 assay. Combination index (CI) values were calculated by Compusyn software. C PANC1 cells were subjected to 40 µM DHA or/and DDP treatment for 24 h and followed by Annexin V-FITC/PI assay. D PANC1 cells were pretreated with 40 µM DHA or/and DDP for 24 h and subjected to the Calcein-AM/PI staining (Calcein AM: live cells, PI: dead cells). Scale bar: 100 µm. E, F The colony formation assay of PANC1 and SW1990 cells were performed under mono or combination treatment of DHA (5 µM for PANC1 or 10 µM for SW1990) and DDP (5 µM for PANC1 or 10 µM for SW1990) for 7 days. The representative images and the corresponding quantitative histograms from three independent experiments are shown (values represented mean ± SD. ★P < 0.05, ★★P < 0.01 versus control).