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. 2021 Jul 15;12(7):705. doi: 10.1038/s41419-021-03996-y

Fig. 3. DHA co-treatment with DDP synergistically impairs mitochondrial homeostasis.

Fig. 3

A, B Observation of the changes in mitochondrial morphology. The treated PANC1 cells were stained with MitoTracker probe (100 nM) and DAPI (10 μg/mL) and then photographed by confocal laser microscope. Scale bars: 10 μm. C, D Mitochondrial oxygen consumption rate (OCR) was carried out with a Seahorse analyzer after the sequential addition of oligomycin, FCCP, and Antimycin A/ Rotenone. The OCR values of maximal respiration, ATP and non-mitochondrial oxygen consumption were normalized to the basal respiration. E, F Flow cytometry was performed to measure mitochondrial ROS by mitoSOX probe (3 μM) (values represented mean ± SD. ★P < 0.05, ★★P < 0.01 versus control).