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. 2021 Jul 14;11:14444. doi: 10.1038/s41598-021-93626-6

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Screening of root cultures for host and non-host potential with respect to the AM symbiosis. (a) Possible point of entry of AM fungus into the root segment (appressorium; depicted by black circle) might be representative of initial contact enabling AM fungal penetration into the roots; (b) Established host culture showing well developed vesicles (V) and collapsing arbuscules (a) connected via hyphal network (white arrow shows the host cells while the black arrow shows AM fungal hypha); (c) Tomato cv. Grafter root cortical cells devoid of any AM fungal structures confirm their inability to sustain the symbiosis, (b) root segments of tomato cv. Grafter that has possible signs of mycorrhizal entry (white arrows) but no markers of successful symbiosis such as well-developed hyphal network, arbuscules or vesicles.