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. 2021 Jul 14;11:14444. doi: 10.1038/s41598-021-93626-6

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Comparison of the number of differentially expressed genes (DEGs) (up and down-regulated) for the non-inoculated host and non-host with AM-inoculated host. Venn diagram represents DEG profiles at FDR ≤ 0.05 and − 2 ≤ log fold change ≥ 2. Genes exclusively expressed in the basal root transcriptome (without any AM inoculation) is represented by the non-overlapping area of the blue circle (RomaNMyc vs GrafterNMyc). Yellow circle (excluding overlap area) represents DEGs in AM-inoculated host (RomaVC2_Gi) compared to non-inoculated non-host (GrafterNMyc). Non-overlapping region of green circle represents DEGs in AM-inoculated host (RomaVC2_Gi) compared to non-inoculated host (RomaNMyc). The 963 genes constituting the overlapping region (light green) in these two sets, are DEGs specific to AM-inoculation in hosts (up- and down-regulation) as these do not overlap with basal root profiles (blue circle). These genes were selected for further characterization.