Table 2.

List and symbiotic-role of known gene candidates (set 2) used for phylogenetics.

Gene name	Role in AM symbiosis	References
LYK10	Lysin motif receptor like kinases, perceiving Myc COs and Myc LCOs pre-contact and required for AM penetration of plant roots	22
LYK12	Required for colonization by AM fungus; belongs to both Solanaceae and Leguminosae species clade	69
CCaMK	Initiator of calcium signalling cascade in hosts; works in accordance with CYCLOPS and DELLA to activate RAM1 required for arbuscule development	56,57
DELLA	Antagonism to GA; involved in regulating the extent of AM symbiosis having synergistic function with CYCLOPS-CCaMK to activate RAM1	16,64
CASTOR	Twin ion channels; required for AM colonization of roots	74
POLLUX
HMGR-CoAa	Initiates the calcium spiking needed for activation of downstream transcriptional responses to AM symbiosis	19,62
CCD7a	Involved in strigolactone biosynthesis important for host perception by AM fungus	79,80
CCD8
SYMRK	Symbiotic receptor-like kinase; required for early signalling events and transduction of signals to activate downstream cascades	54,55
CNGC15	Required for nuclear localised symbiotic calcium oscillations	81
CYCLOPS/IPD3b	Required for arbuscule initiation; part of the CCaMK-DELLA-CYCLOPS complex activating many transcription factors required for symbiotic association including RAM1	11,57

^aLiterature candidates found in transcriptomics data.

^bAbsent in non-host proteomes (not used in further analysis).