Fig. 2. Detection of intracellular dsRNA and dsDNA initiates accumulation of LDs in multiple cell types.

a Primary immortalised human astrocyte cells stimulated with dsRNA and dsDNA tagged with Rhodamine (red) for 8 h and stained with Bodipy (493/503) to visualise LDs (green) and DAPI to visualise the cell nuclei (blue). Cells were imaged on a Nikon TiE microscope. Original magnification is 60X. Scale bar, 50 μm. b Average number of LDs per cell and (c) average LD sizes (diameters) were analysed from greater than 200 cells in a range of cell types, using ImageJ analysis software (n = 2 biological replicates). d LD size distribution in primary immortalised astrocyte cells stimulated with either dsDNA or dsRNA for 8 h. Image represents a single astrocyte cell following dsRNA stimulation. Cells were stained with Bodipy (493/503) to visualise LDs (green) and DAPI to visualise the cell nuclei (blue) and LD size distributions were analysed on ImageJ analysis software. Scale bar, 15 μm. In (b–d) error bars, mean values ± SEM, P values were determined by unpaired two-tailed Student’s t test with a Holm-Sidak correction for multiple comparisons (greater than 300 cells; n = 3 biological replicates). Stimulated cells were statistically compared with their respective mock controls, ns = not significant. Source data are provided as a Source Data file.